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p38 Mitogen-Activated Protein Kinase Affects Transforming Growth Factor-beta/Smad Signaling in Human Dental Pulp Cells.

Wang FM, Hu T, Tan H, Zhou XD

Key Laboratory of Oral Biomedical Engineering Ministry of Education, Sichuan University, Chengdu, China, hxkq@mail.sc.cninfo.net.

Transforming Growth Factor-beta (TGF-beta) plays an essential role in differentiation of dental pulp cells into odontoblasts during reparative dentine formation. However, the mechanism by which TGF-beta stimulates dental repair remains rather obscure. Human dental pulp cells were used as an in vitro model in the present work. We showed that TGF-beta signaled through mitogen-activated protein kinases (MAPKs), such as ERK1/2 and p38, along with Smad pathway. Distinct pathways exerted different time response. SB203580, a specific p38 MAPK inhibitor, reduced phosphorylation of Smad3, while it slightly enhanced phosphorylation of Smad2. Increased phosphorylation of ERK1/2 and p38 confirmed that SB203580 did not block activation of TGF-beta receptors. In addition, the inhibition of ERK1/2 activity with MEK1/2 inhibitor U0126 increased TGF-beta mediated phosphorylation of Smad3. Our results suggest that p38 affects the phosphorylation of Smad2 and Smad3 differentially during TGF-beta signaling in human dental pulp cells and ERK1/2 might be involved in the process.

Published 12 October 2006 in Mol Cell Biochem, 291(1): 49-54.
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